Capturing Transient & Dynamic Interactions to Characterize Intrinsically Disordered Proteins
- Explore the fundamental challenges of studying proteins that lack a fixed 3D structure and how conformational heterogeneity and how it complicates affinity determination and stoichiometry
- Discuss approaches like NMR, HDX-MS, SAXS, smFRET, and cryo-EM for resolving structural ensembles and learn which is best suited to detect weak and transient interactions
- Learn strategies to interpret biophysical data when no single static structure exists