Quantifying Reversible & Irreversible Interactions of Covalent Small Molecule Binders to Non-Enzyme Targets Using Biophysical Methods

  • Explore the considerations for use of different techniques (MS, FRET, SPR, spectral shift) to measure compound potency through kinetic measurements
  • Discuss how to implement experimental setups and kinetic modeling for characterization of covalent kinetics by SPR
  • Present how information from orthogonal techniques can be combined for greater understanding of covalent compound interactions