Despite advances in high-resolution and high-sensitivity techniques, many biophysical assays still struggle to accurately reflect the complexity of physiological environments. This roundtable allows you to openly discuss with your industry colleagues the persistent challenge of bridging purified, reductionist assay data with meaningful biological and in vivo outcomes.

Participants will explore:

• Where reductionist assays fall short: common sources of artefacts, over-simplification, and false confidence when moving from purified systems to native biological matrices
• Practical strategies to increase physiological relevance: working with biofluids, complex media, membrane mimetics, or intact protein assemblies
• Translational alignment: how teams correlate SPR/ITC/HDX/native MS/focal molography data with cellular, phenotypic, or in vivo readouts
• Operational realities: assay design trade-offs, throughput vs. realism, material constraints, and when “good enough” biophysical insight is truly enough
• Future opportunities: what technologies, workflows, or cross-disciplinary partnerships could help close the reductionist–physiological gap