Unleashing the Power of Spectral Shift Technology for Ultra-High Throughput Binding Assays
Time: 12:00 pm
day: Day 1
Details:
– High-throughput screening (HTS) is vital in drug discovery, yet traditional methods like biochemical assays and ASMS face potential limitations.
– We established a spectral shift-based direct binding assay that enhances precision, sensitivity, and efficiency while reducing sample consumption and turnaround time.
– This approach has been successfully applied in ultra HTS against targets such as kinase BTK, CDK2, PIK3CA, and transcription factor STAT6.
– Additionally, we developed a high-throughput method to determine Kinact/Ki for non-reversible interactions, offering superior sensitivity and throughput compared to traditional orthogonal methods, and showcased its application in characterizing covalent fragment binding to BTK.
